Abstract

IN-VITRO EVALUATION OF ANTI-UROLITHIASIS POTENTIAL OF KARUVANGA CHENDOORUM BY STRUVITE CRYSTAL GROWTH INHIBITION ASSAY

K. Anbarasan*, C. Sasikala, D. Sivaraman and P. Shanmugapriya

ABSTRACT

Urolithiasis is a kind of metabolic disorder were individual’s biological system is unable to maintain the calcium hemostasis which was characterized by the formation of stone in the kidney, bladder or urethra. Still, now there is no proper treatment available for clinical management of this condition and there are no proper drugs available in modern medicine that can correct the altered physiology of hemostasis. Siddha system of medicine comprises of natural remedy with therapeutic effects that can dissipate and remove even the larger stones would eliminate the need for a surgery and the risks associated with it. Karuvanga chendoorum (KC) is one such novel Siddha formulation presently used for the treatment of Pramiyam(syphilis), Perumpadu (leucorrhea) and Neerizhivu (diabetic). The main aim of the present investigation is to carry out the anti-urolithiasis activity of the formulation KC by struvite crystal growth inhibition assay at two dose level of 0.5% and 1%. The efficacy of the KC was evaluated by comparing the crystal size of treated medium with that of the control. The average size of the crystal was higher in the control medium with the length of 1.44 ± 0.08 cm and the size of the crystal was significantly decreased in medium contains 0.5% and 1% of test drug KC with the average length of 0.98 ± 0.13 and 0.58 ± 0.17 cm. From the result of the study, it was concluded that the test drug KC has Promising anti-urolithiasis property in the tested medium.

Keywords: Urolithiasis, Siddha system, Karuvanga chendoorum, Struvite crystal, Kidney, Calcium hemostasis.