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World Journal of Pharmacy and
Pharmaceutical Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology

Impact Factor : 8.025

ICV : 84.65

WJPPS Citation

	All	Since 2019
Citation	5450	3969
h-index	23	20
i10-index	134	84

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WJPPS: NOVEMBER ISSUE PUBLISHED

NOVEMBER 2024 Issue has been successfully launched on 1 NOVEMBER 2024.

Abstract

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR DETERMINATION OF TENOFOVIR DISOPROXIL FUMARATE IN BULK AND DOSAGE FORM

Yogesh P. Pawar*, Shaadmin N. Shaikh and Pratap S. Dabhade

ABSTRACT

Objective of the present analytical research work was to developed and validate RP-HPLC method for the estimation of Tenofovir Disoproxil Fumarate in bulk and dosage form. Method & Results: The RP-HPLC method for Tenofovir Disoproxil Fumarate was developed using column C-18 (4.6 x 250 mm) as stationary phase Methanol: Water (0.1 %OPA (85:15 v/v) as mobile phase. The mobile phase was maintained at a flow rate of 0.7 ml/min, Temperature is Ambient, HPLC System- Younglin (S.K) Gradient System UV Detector, system- Autochro - 3000. The method was validated in accordance with ICH guidelines. Conclusion: The developed HPLC method was simple, rapid, easy, accurate and precise. So, the method can be applied successfully for the routine analysis of Tonofovir Disoproxil Fumarate in pharmaceutical industry.

Keywords: Tonofovir Disoproxil Fumarate, Methanol, Water, RP- HPLC, Method Development and Validation.

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