Abstract

ANTIOXIDANT ACTIVITY OF DIFFERENT FRACTIONS OF METHANOLIC EXTRACT OF YOUNG SHOOTS OF BAMBUSA ARUNDINACEA

Kajal N. Chauhan, Bhavini Shah and Dr. Anita A. Mehta*

ABSTRACT

Background: Bambusa arundinacea (Bamboo) belonging to family (Graminae) is used in number of disease including liver ailments in various parts of India. The present study was done to evaluate antioxidant activity of different fractions of methanolic extract of young shoots of Bambusa arundinacea. Material and method: Antioxidant activity of different fractions(n-hexane, chloroform, ethylacetate and n-butanol) of methanolic extract was evaluated by DPPH, Lipid peroxidation and Ferric reducing power assay. The Total Phenolic (TPC) and Total Flavonoid contents (TFC) of all the fractions were also determined. Results: The results of the present analysisdemonstrated that different fractions of methanolic extract of young shoots of Bambusa arundinacea contained appreciable levels of total phenolic and flavonoid contents and also possess good antioxidant activity. Of all the fractions tested, ethylacetate fraction was found to be more effective with total phenolic content (30.8±1.5 mg gallic acid equivalent (Gallic acid)/g DW) and total flavonoid content (47±2.6 mg Quercetin equivalent/g DW). Conclusion: The results of the present study shows that different fractions of methanolic extract of young shoots of Bambusa arundinacea are a viable source of natural antioxidants and might be exploited for functional foods and nutraceutical applications.

Keywords: Bambusa arundinacea, DPPH, Antilipid peroxidation activity, Reducing power assay.