PRELIMINARY PHYTOCHEMICAL SCREENING AND HPTLC FINGERPRINT ANALYSIS OF CHLOROFORM EXTRACT OF POLYHERBAL FORMULATION
Sumia Fatima*, Dr. C. Ramesh and Abdul Saheel Qureshi
ABSTRACT
The aim of the study is to establish the HPTLC fingerprint profile and phytochemical screening of chloroform extract of Polyherbal formulation AET. The solvent system selected for HPTLC was Toluene: Ethyl Acetate: Methanol (7:2:1) v/v, stationary phase was precoated aluminium plate of silica gel 60 F254 (10X10cm) with 0.2mm thickness. The separated bands on the HPTLC plates were scanned under UV at 254nm, 366nm and visible 580nm (Iodine vapours). HPTLC fingerprinting analysis of the Polyherbal formulation AET showed the presence of possible number of components.
Keywords: Polyherbal formulation AET, Chloroform extract, HPTLC fingerprint, Solvent system.
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