Abstract

POTENCY EVALUATION OF COMBINED THERAPY OF LOSARTAN AND ANANAS COMOSUS DURING ISOPRENALINE MEDIATED CARDIAC DYSFUCNTION IN RATS

Sushma Attuluri*, Syed Inayath Hussain, Dr. Mohsina Hassan, Urooj Fatima, Syeda Amtul Aleem Afifa, Amtul Aziz Afia

ABSTRACT

The present study was carried out to explore the cardioprotective potency of pineapple (ananas comusus) with losartan in isoprenaline(ISO) induced acute and chronic myocardial damage in rats. The purpose of the study research was to uncover the potency of anti inflammatory activity of pineapple on necrotic myocardium, in combination with angiotensin blocker losartan(LTN). In acute model, Sprague Dawley rats were pre-treated with losartan(LTN) (30 mg/kg, ten days, po), low,intermediate and high doses of pineapple (100, 250 and 500 mg/kg, 30 days, po) and subsequently subjected to ISO administration (150 mg/kg, s.c) for two consecutive days. The influence of prophylactic treatment was analysed by quantification of biomarkers, antioxidants and histopathological observations. LDH and CK-MB activities were studied in serum and heart tissue homogenate (HTH) where as SOD, catalase and TBARS were estimated in HTH. There was significant elevation in LDH and CK-MB gactivities in HTH of all treated grups as compared to ISO control.SOD and catalase activities were also significantlt increased in treated groups. In chronic model, ISO (3 mg/kg/day, s.c) was administered to Sprague Dawley rats treated with low,intermediate and high doses of pineapple (100, 250 and 500 mg/kg, p.o. thirty days) and LTN (30 mg/kg, p.o. ten days). Through retro orbital route blood is withdrawn for LDH and CK-MB estimations. Antioxidants were estimated by biochemical method and histopathological studies were carried out to support biochemical studies. Prior treatment of animals with Pine apple and LTN resulted in elevation of biomarker activities in heart tissue homogenate and decline in serum. The SOD and Catalase activities were significantly elevated with depletion of TBARS and histological scores in groups subjected to prophylactic treatment. Among various treatments, high dose of Pine apple and LTN was found to be most effective. From the present results it may be concluded that high dose of Pine apple (500 mg/kg) possess cardioprotective efficacy when given prophylactically in experimental animals. However, further studies should be carried out to explore the ultrastructural changes and underlying mechanisms of protection when these substances are used together.

Keywords: Isoprenaline, Losartan, Pine Paple, Myocardial Damage, LDH, CK-MB, SOD, Catalase, TBARS.