Abstract

EXPLORATION OF THE POTENTIAL L- ASPARAGINASE PRODUCING BACTERIA FROM SOIL ITS ATTRIBUTES AS AN ANTIOXIDANT ACTIVITY

*Naveen Banu M.

ABSTRACT

In recent years, microbial L-asparaginases have drawn particular attention because of their potential antineoplastic properties and significant application in food industries. The present study was undertaken to establish microbial species that produces L-asparaginase, which might have even more efficient antineoplastic activity. Three bacterial isolates were screened for the production of L-asparaginase on a Modified M9 Agar Medium with phenol red as an indicator. Quantitative estimation was done by Nesselerisation. However, three bacterial isolates showed highest enzyme activity exhibiting a pink zone of hydrolysis of around 3.0 mm. Among all positive asparaginase producing strains were identified as Escherichia coli, Klebsiella pneumonia and Pseudomonas aeruginosa on the basis of morphological and biochemical characterization. Among them the most significant activity was shown by Pseudomonas species (471.6 IU/mg). Good scavenging property and the ability to lower the acrylamide levels in food stuffs makes it a valuable enzyme in pharmaceutical and food industries.

Keywords: L-asparaginase, Production, Purification and Antioxidant activity.