Abstract

ANTIHYPERLIPIDEMIC ACTIVITY OF HEMIDESMUS INDICUS BIOFRACTION IN POLOXAMER-407 INDUCED HYPERLIPIDEMIC RATS

Mehul G. Rana*, Dr. Ashvin V. Dudhrejiya and Rachana V. Katbamna

ABSTRACT

The various bio-fractions of water extract of roots of Hemidesmus indicus (family: Asclepiadaceae) was evaluated for antihyperlipidemic activity in poloxamer 407 induced hyperlipidemic rat. Hyperlipidemia was induced in rat by i.p. injection of poloxamer 407 (30% w/w in distilled cool water; 400 mg/kg) and 1 hours before the administration of P-407, the rat of reference group were administered with atorvastatin (50 mg/kg p.o.) and finofibrate (FF 65 mg/kg p.o.) while test groups received the various bio-fractions (Benzene, Ethyl acetate, Methanol, Water) of water extract of plant (100 mg/kg p.o and 200 mg/kg p.o of each bio-fractions). After 3, 6 and 24 hour of treatment, serum lipid profiles were investigated. The administration of the ethyl acetate fractions of root extract significantly (p < 0.05) reduced the serum levels of total cholesterol, triglycerides (TG) and very low density lipoprotein (VLDL) as well as the atherogenic index (A.I.) the P-407 induced hyperlipidemic control rats after 6 h of treatment. After 24 h of treatment, the ethyl acetate fractions of extract induced a significant reduction (p < 0.001) in serum total cholesterol (TC), VLDL, low density lipoprotein (LDL) as well as the atherogenic index and significant increase in HDL levels, when compared to P-407 control group. All these effects were comparable to those of the reference standard, atorvastatin and finofibrates. The results of the investigation was found to be that the ethyl acetate fraction water extract of Hemidesmus indicus has potential antihyperlipidemic activity and might be used for the prevention of hyperlipidemia associated disorders.

Keywords: atherogenic index, hyperlipidemia, Hemidesmus indicus, bio-fractions, poloxamer 407.