Abstract

ANTIHYPERLIPIDEMIC AND ANTIOXIDANT ACTIVITY OF BIOFRACTIONS OF FLOWERS OF HIBISCUS ROSA-SINENSIS IN POLOXAMER 407 INDUCED HYPERLIPIDEMIC RATS

Mehul G. Rana*, Dr. Ashvin V. Dudhrejiya and Rachana V. Katbamna

ABSTRACT

Hyperlipidemia is considered to be one of the greatest risk factors contributing to the prevalence and severity of cardiovascular diseases. Hyperlipidemia was induced by poloxamer 407 (30% w/w in distilled cool water; 500 mg/kg, i.p). Prior to the administration of P-407, the rat of reference group were administered with atorvastatin (50 mg/kg p.o.) and finofibrate (FF 65 mg/kg p.o.) while test groups received 1 hr before dose (100 mg/kg p.o and 200 mg/kg p.o of each bio-fractions) of various bio-fractions (Benzene, Ethyl acetate, Methanol, Water) of methanolic extract of plant. Serum lipid profiles were investigated after 3, 6 and 24 hour of treatment. The result were found that ethyl acetate fractions of methanolic extract significantly reduced the serum levels of total cholesterol (TC) (p < 0.05), triglycerides (TG) (p < 0.001) and very low density lipoprotein (VLDL) (p < 0.001) as compare to the disease control rats after 6 h of treatment. After 24 h of treatment, the ethyl acetate fractions of extract induced a significant reduction in serum TC (p < 0.001), TG (p < 0.001), VLDL (p < 0.001), low density lipoprotein (LDL) (p < 0.05) and significant increase in high density lipoprotein (HDL) levels (p < 0.001), the benzene fractions of extract induced a significant reduction in serum TC (p < 0.05), TG (p < 0.01), VLDL (p < 0.01) and not significantly reduced LDL (p < 0.05) and not significant increase in HDL levels, when compared to disease control group. All these effects were comparable to those of the reference standard, atorvastatin and finofibrates.

Keywords: Antihyperlipidemia, Hibiscus rosa-sinensis, Cholesterol, Poloxamer 407.