Abstract

ANTIBACTERIAL ACTIVITY OF METHANOLIC EXTRACT OF MANGIFERA INDICA (BARK) AND OSYRIS LANCEOLATA (LEAVES) FROM WESTERN REGION OF NEPAL

P. S. Bhandari, R. Bhandari, B. K. Sah, S. Gyawali, M. Bhusal, S. Shakya*, Dr. Sunil Shrestha

ABSTRACT

Nature has been a source of medicinal agents since times immemorial. The importance of herbs in the management of human ailments cannot be over emphasized. Different extracts from traditional medicinal plants have been tested to identify the source of the therapeutic effects. As a result some natural products have been approved as new antibacterial drugs. The objective of this research is to find out the potent antibacterial agents from the selected medicinal plants: Mangifera indica and Osyris lanceolata of Rupandehi, and Palpa districts of Nepal. The Bark and Leaves of the plants Mangifera indica and Osyris lanceolata respectively were subjected to extraction bymaceration process using solvent methanol. Screening of antibacterial activity was done by disc diffusion method against the microorganisms Staphylococcus aureus and Escherichia coli at three different concentrations (1 mg/disc, 2 mg/disc and 4 mg/disc). Both plants showed potent antibacterial activity. Minimum inhibitory concentration (MIC) assay were determined for the two extracts against these bacterias. The concentration 4 mg/disc of M. indica showed maximum activity against both E. coli and S. aureus i.e., 11.16 ± 0.28 and 11.33 ± 0.29 respectively. Although both plants have exhibited best results against gram positive as well as gram negative bacteria, the crude extract of these plants further needs to be purified through antibacterial activity guided fractionation to isolate and identify the compounds responsible for the antibacterial activity. So, this study provides the platform to carry out further researches to give light upon specific compounds which are responsible for their antibacterial activities.

Keywords: Antibacterial, Staphylococcus aureus, Escherchia coli, Methanol, Disc Diffusion Method, Minimum Inhibitory Concentration, Mangifera indica and Osyris lanceolata