Abstract

COMPARISON OF ANTIBACTERIAL AND PHYTOCHEMICAL EVALUATION OF EXTRACTS FROM LEAVES OF ORGANIC AND NON ORGANIC CAMELIA SINENSIS

Khadka Dipendra*, Aryal Pramod, Khanal Rita, Gautam Narayan and Shah Anita

ABSTRACT

The research was conducted to compare the antibacterial and phytochemical evaluation of extracts from leaves of organic and non organic Camelia sinensis. Based on the cultivation Camelia sinensis is broadly classified into two types: Organic Camelia sinensis and Non organic Camelia sinensis. Organic tea is free of synthetic fertilizer, herbicides and pesticides and the people are attracted to the organic tea. Phytochemical analysis were done with the established method and total phenolic content (TPC) was determined by using Folin Ciocalteu colorimetric method.The antibacterial activity of methanol and chloroform extracts(100 mg/ml and 200 mg/ml) of both organic and non organic tea were compared against gram positive strain Staphylococcus aureus(ATCC-25923) and gram negative strains Escherichia coli(ATCC- 25922). The Zone of Inhibition (ZOI) of extracts was compare with ampicillin 10μg/disc for gram positive bacteria whereas gentamicin 10μg/disc for gram negative bacteria. The results indicated that the TPC of methanol and chloroform extracts from organic garden was far better than the non organic garden Camelia sinensis..The antibacterial results also showed that the extracts from organic Camelia sinensis were better than to that of non organic Camelia sinensis. The results revealed that the antibacterial action of extracts against Escherichia coli is better in chloroform extracts whereas the antibacterial action of methanol extracts is better in Staphylococcus aureus than Escherichia coli.

Keywords: Phytochemical, Total Phenolic Content, Antimicrobial Screening.