Abstract

EFFICACY OF A POLYHERBAL FORMULATION MADHUMEHARI IN ATTENUATING DIABETIC NEPHROPATHY.

Reema Mitra*, Papiya Mitra Mazumder, Upendra K. Jain

ABSTRACT

Oxidative stress in kidneys increases during diabetic nephropathy. Polyherbal formulation Madhumehari was assessed for its ability to decrease oxidative stress in kidney homogenates of diabetic rats. Animals were rendered diabetic by a single i.p. injection of 60 mg/kg b.w. streptozotocin. After 72 hours of STZ injection blood glucose was estimated. The animals having fasting blood sugar above 200mg/dl were included in the study. Treatment was started 7 days after the induction of diabetes. The animals were divided into the following groups with 6 rats in each group: Group –I: Normal control rats. Group –II: Diabetic control Group-III: Diabetic animals received Madhumehari at a dose of 500 mg/kg b.w. Assessment of renal oxidative stress: The kidneys were isolated from each animal. The renal cortical homogenates were centrifuged at 5000 rpm for 10 min at 4â—¦C. The resulting supernatant was used for determination of:  Malondialdehyde (MDA)  Catalase  Superoxide Dismutase (SOD)  Glutathione –S- Transferase Treatment with Madhumehari showed significantly decreased MDA level and GST level showing a reversal of peroxidation. SOD and Catalase level was significantly increased as compared to the diabetic control group. The results of the study indicate that Madhumehari was beneficial in decreasing the oxidative stress in the treatment group as compared to the diabetic control group. Thus Madhumehari may be useful in preventing renal complications in diabetic patients.

Keywords: Diabetic Nephropathy, Madhumehari, Oxidative stress, In –vivo antioxidant enzymes.