Abstract

VALIDATION STUDY FOR SIMULTANEOU ESTIMATION OF ESCITALOPRAM OXALATE AND FLUPENTHIXOL HYDROCHLORIDE IN TABLET DOSAGE FORM BY HPLC

Patel Nilam J.*, Bhumi Patel, Kaushik Patel, Jaymin Patel, Yogesh Patel and Komal Siddhanti

ABSTRACT

A simple, rapid, economical, precise and accurate Stability indicating RP-HPLC method for simultaneous estimation of Escitalopram Oxalate and Flupenthixol Hydrochloride in their combined dosage form has been developed. A reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of Escitalopram Oxalate and Flupenthixol Hydrochloride. In Their Combined Dosage form has been developed. The separation was achieved by LC- 20 AT C18 (250 mm x 4.6mm, 5 μm) column and Buffer (pH 6.5): Acetonitrile (50:50%v/v) as mobile phase, at a flow rate of 1 ml/min. Detection was carried out at 256 nm. Retention time of Escitalopram Oxalate and Flupenthixol Hydrochloride were found to be 3.423 min and 6.163 min, respectively. The method has been validated for linearity, accuracy and precision. Linearity observed for Escitalopram Oxalate (10-30μg/ml) and for Flupenthixol Hydrochloride( 0.5-1.5 μg/ml). Developed method was found to be accurate, precise and rapid for simultaneous estimation of Escitalopram Oxalate and Flupenthixol Hydrochloride.

Keywords: Escitalopram Oxalate, Flupenthixol Hydrochloride, Stability indicating RP-HPLC Method.