Abstract

SPECTROPHOTOMETRIC ASSAY OF TENELIGLIPTIN IN PHARMACEUTICAL TABLET DOSAGE FORM BY ION-PAIR COMPLEX METHOD USING BROMO PHENOL BLUE (BPB)

I. Lakshmi Prasanna*, Nuzhath Fathima, G.T. Naidu, I. E. Chakravarthy and P. Raveendra Reddy

ABSTRACT

A simple Ultraviolet Spectrophotometric method has been developed for the determination of Teneligliptin in pharmaceutical formulations and in the tablet dosage form using Bromo Phenol Blue (BPB). The interaction of the drug with BPB results in the formation of an ion- pair complex the composition of which is established as 1:1 (Drug: BPB) by Job’s continuous variation method. The wavelength of maximum absorbance of the Ion-pair complex is found to be 305 nm. The absorbance values of the Ion-pair complex are found to increase linearly with the increase in the amount of the drug Teneligliptin up to 300 μg/ml. This suggests the suitability of the method for the determination of the drug in the range 10 μg/ml to 300 μg/ml. This also shows the verification of the Beer-Lambert’s Law in this range. The method is successfully employed to evaluate the assay of commercial tablets in pharmaceutical formulations for Teneligliptin and the results agreed very well. The molar absorptivity and Sandell Sensitivity of the method are 1.877 x 104 lit. mol-1. cm-1 and 0.02273 μg.ml-1.cm2 respectively.

Keywords: Spectrophotometry, Teneligliptin, Ion-pair complex, Bromo Phenol Blue, Pharmaceutical formulations.