Abstract

ANTIBACTERIAL EFFECTS OF AERIAL PART OF BARLERIA PRIONITIS WITH ETHANOL EXTARCT ON PATHOGENIC STRAINS

Priyanka Kumari Panchal*, Nishi Sharma and Kamal Singh

ABSTRACT

study was planned for the investigation of antimicrobial effects of Barleria prionitis leaves. Therefore, the preliminary successive solvent extraction and chemical test revealed the presence of secondary metabolites in various extracts. Microbial inhibitory effects can provide us clue for further investigation. Ethanol extract was used for investigation of inhibition on five pathogenic bacterial strains and done by broth dilution methods with triplicate. Nutrient media was used for bacterial culture. Tetracycline was used as positive control and double distilled water was used as Negative control. Statistical analysis has been done by Mean ± Standard Deviation with range, in which n=3. There were five bacteria were tested, out of which drug has been givenbest results against P. aeruginosa while average effect against B. thruengenesis, C. diphtheria, S. typhi, and C pneumonia and the plant leaves have shown the presence of alkaloids, flavanoids, saponins and tannins. As we calculate that Barleria leaf ethanol extract diluted with 2.5 mg/ml to get 214.4 activities against B. thruengenesis, S.typhi and C. diphtheria but in 5 mg/ml dilution 107.2 activities has been get against, C.pneumoniae while for P. aeruginosa 1.25 mg/ml dilution given 428.8 Total activities. The highest activity of our study is 428.8. In the present study, we found that Barleria prionitis Leaf ethanol extract was given the very best inhibitory effect against all tested pathogenic bacteria. But B. thruengenesis, C. diphtheria and Salmonella typhi were very sensitive with Barleria leaves. Barleria leaves with ethanol extract given the very best results against Pseudomonas aeruginosa. It has been given the presence of flavonoids, alkaloids, saponins and tannins, which is responsible to show antimicrobial, relieving in toothache and whooping cough. Barleria leaves have high level of activity against tested pathogenic strains.

Keywords: Barleria prionitis, secondary metabolites, antimicrobial activity, Ethanol, Total activity, statistical analysis, Phytoconstituents.