Abstract

STABILITY-INDICATING SIMULTANEOUS ESTIMATION OF VILDAGLIPTIN AND MIRABEGRON IN BULK AND PHARMACEUTICAL DOSAGE FORM BY USING UV SPECTROSCOPY

G. Raveendra Babu*, G. Vijay Kumar, M. Kalyani, Md. Roshna, P. Jeevana Rani, P. Vijay Kumar and S. Ajay

ABSTRACT

The present study was stability-indicating simultaneous estimation of Vildagliptin and Mirabegron in bulk and pharmaceutical dosage form. The detection, wave length of Vildagliptin at 204 nm using water used as a solvent and detection of wavelength at 245 nm using phosphate buffer pH is 6.8 used as a solvent. Two drugs obey beer’s law concentration range of 10-100 μg/ml for Vildagliptin and concentration range of 1-10 μg/ml for Mirabegron, respectively. The relative standard deviation of both intra-day and inter-day precision were less than 2% found in Vildagliptin and Mirabegron. The LOD and LOQ were found to be 0.834 μg/ml and 2.52 μg/ml for Vildagliptin and found to be 7.67 μg/ml and 23.24 μg/ml for Mirabegron, respectively. The recovery of Vildagliptin and Mirabegron in tablet dosage form was found within the limits. Performing the stability indicating studies under different conditions found it’s negligible in both drugs. The present method was successfully adopted for the estimation of Vildagliptin and Mirabegron in bulk sample preparations.

Keywords: Vildagliptin, Mirabegron, Simultaneous, Estimation, Ultra violet, Stability.