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Abstract

DEVELOPMENT AND VALIDATION UV- VISIBLE SPECTROPHOTOMETRIC METHOD OF ESTIMATION OF TERBUTALIN SULPHATE IN BULK DRUG BY USING NINHYDRIN.

Shital Godse*, Gajanan A. Vaishnav, Sushama Vaishnav-Hota, Angadi S.S., Patil S. J.

ABSTRACT

An accurate, precise and simple spectrophotometric method for the determination of Terbutalin sulphate based on the formation of a yellow color product with Ninhydrin in the presence of sodium bicarbonate with an λmax at 346 nm. The Reaction heating temperature is 80°c in 30 min. The calibration curve is linear over the range of 10-60 μg/ml and the regression equation y=0.0263x+0.2with a regression coefficient (r) of 0.98 (n=10).The limit of Detection (LOD) and limit of Quantification (LOQ) calculated as per ICH guidelines are 18.15μg/ml and 55μg/ml, respectively.

Keywords: Terbutalin sulphate, UV-Spectrophotometer, Ninhydrin.


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