Abstract

IN SILICO MICROARRAY DATA ANALYSIS OF OVER-EXPRESSED ECCC GENE RESPONSIBLE FOR TUBERCULOSIS

Talat Khan* and Sazid Ali

ABSTRACT

Tuberculosis (TB) is an infectious disease that is caused by a bacterium called Mycobacterium tuberculosis. TB primarily affects the lungs, but it can also affect organs in the central nervous system, lymphatic system, and circulatory system among others. The disease was called "consumption" in the past because of the way it would consume from within anyone who became infected. Microarray data analysis is used to get the co-regulated genes along with the gene expressions. Co-regulated genes may share similar expression profiles, may be involved in related functions or regulated by common regulatory elements. There are different approaches to analyze the large-scale gene expression data in which the essence is to identify gene clusters. This approach has allowed us to (a) determine expression profiles of previously described developmentally regulated genes: in this work raw data of patients infected by Tuberculosis is used as a sample, (b) identify novel developmentally regulated genes; here co-expressed genes of these three over-expressed genes were analyzed by using Insilico approaches such as clustering (HCL, KMC) and phylogenetic analysis.

Keywords: Tuberculosis; Microarray data analysis; Insilico; gene expression; phylogenetic analysis.