Abstract

SYNTHESIS OF PLANT-MEDIATED SILVER NANOPARTICLES USING COMMIPHORA WIGHTII (GUGGUL) EXTRACT AND STUDY THEIR ANTIBACTERIAL ACTIVITIES AGAINST FEW SELECTED ORGANISMS

Diptendu Sarkar* and Goutam Paul

ABSTRACT

The primary objective of this investigation is to green synthesis of silver nanoparticles by using Commiphora wightii leaves extracts and to evaluate their antimicrobial activity against few selected organisms. The original suspension of plant extracts was yellowish-green in colour. However, after addition of AgNO3 within 20mins, the suspension showed colour change and turned dark brown after 5 hrs of incubation at room temperature. UV-Vis spectral analysis in the range of 200-600 nm, confirmed the formation of silver nanoparticles. It is a well-known, that silver ions and nanoparticles are very much potential antimicrobial molecule as they possess many inhibitory and bactericidal effects and so it can be used as an antibacterial agent. The antibacterial activity of silver nanoparticles is proved by the zone of inhibition in nutrient agar media. The silver nanoparticles biosynthesized by Commiphora wightii plant leaves extracts showed antibacterial activity against three selected Gram Negative organisms and one Gram Positive ogranisms such as Escherichia coli, Pseudomonas aeruginosa, Baccillus subtilis and Staphylococcus aureus. However, the antimicrobial effect varied with the variation in salt concentration. Additionally, the silver nanoparticles by Commiphora wightii leaves extracts showed good inhibition activity towards Escherichia coli and Pseudomonas aeruginosa. The use of silver nanoparticles in drug delivery systems might have good future.

Keywords: Antimicrobial, Green-synthesis, Bactericidal effect, Leaf extract, Silver Nanoparticles.