Abstract

SEPARATION AND SIMULTANEOUS DETERMINATION OF PROLINE AND ENOXACIN IN HUMAN URINE BY CAPILLARY ELECTROPHORESIS WITH ELECTROCHEMILUMINESCENCE

Xiao Guo, Ming Su* and Hanwen Sun

ABSTRACT

The coupling of Ru(bpy)32+ based electrochemiluminescence(ECL) detection with capillary electrophoresis(CE) was developed for the simultaneous determination of proline and enoxacin in human urine. The effects of experimental conditions including the mode of applied voltage signal, the potential of working electrode, pH value, the flow rate of carrier of solution and the concentration of Ru(bpy)32+ and the ECL intensity of the drugs were investigated in detail. Parameters related to the separation and detection were investigated and optimized. Under optimized conditions the proposed method displayed a linear range from 0.2 to 80 μg mL–1 for proline and 0.1 to 100 μg mL–1 for enoxacin. Their limits of detection were 0.06 and 0.02 μg mL–1, respectively. A baseline separation for proline and enoxacin was achieved within 10 min. Developed method was successfully applied to determine proline and enoxacin in human urine.

Keywords: Electrochemiluminescence; Capillary electrophoresis; Proline; Enoxacin; Human urine.