Abstract

HPLC AND HPTLC SIMULTANEOUS DETERMINATION METHODS OF TRICLABENDAZOLE AND MOXIDECTIN IN COMBINED DOSAGE FORMS

Nageh Abotaleb, Tamer Nasr, Heba Shabaan* and Zeinab Elsherif

ABSTRACT

This manuscript describes two simple validated chromatographic methods for simultaneous determination of Triclabendazole (TCL) and Moxidectin (MOX) in combined dosage forms with no prior separation or interference from excipients. The first method was an isocratic HPLC method on a BDS phenyl C18 column using acetonitrile: methanol: 5mM ammonium dihydrogen phosphate solution (60:30:10, by volume) as a mobile phase at wavelength 242nm, retention times were found to be 1.9 min and 3.9 min for TCL and MOX, respectively. The second method was a simple HPTLC method where separation was performed on HPTLC silica gel 60 F254 plates using ethyl acetate: toluene: formic acid 85%: (50:45: 5, by volume) as a developing system, the developed bands were scanned at 242nm, Rf values were found to be 0.60 and 0.90 for TCL and MOX, respectively. The linear ranges of the first method were found to be 1-200 μg/mL and 0.5-100 μg/mL, while those of the second method were found to be 0.5 -20 μg/band and 0.1-2 μg/band for TCL and MOX, respectively. Both methods were validated and applied for the determination of the two drugs in pure raw material and combined dosage form with no interference from reported excipients and were found to be suitable for quality inspection of combined dosage forms.

Keywords: HPLC, HPTLC, Triclabendazole, Moxidectin.