Abstract

FORMULATION DEVELOPMENT AND IN-VITRO EVALUATION OF ACETAZOLAMIDE SELF MICROEMULSIFYING DRUG DELIVERY SYSTEM (SMEDDS) TO IMPROVE THE SOLUBILITY AND BIOAVAILABILITY

Tarkeshwar Prasad Shukla*, Dr. Ashish Kumar Sharma

ABSTRACT

Self micro emulsifying drug delivery system (SMEDDS) is a novel approach for the formulation of drug compounds with poor aqueous solubility. Acetazolamide SMEDDS were prepared and evaluated. Following emulsification, the optimized formula was selected to have the smallest mean particle size and the highest absolute Zeta potential, which should yield the formation of a stable emulsion. Its dissolution characteristics were superior to the other SMEDDS formulas. The in vitro dissolution indicated a significant improvement in Acetazolamide release characteristics. The preformulation study of drug was carried out initially in terms of physical appearance, melting point, solubility study, λ max determination (wavelength at maximum absorbance) solubility study, and results directed for the further course of formulation. The formulations were prepared with different ratios of oil, surfactant and co-surfactant. The SMEDDS were prepared and are evaluated in terms of droplet size, drug release etc. NIP C1 & NIP C8 selected as optimized batches showed promising results. Zeta potential of NIP C8 indicates good stability. NIP C1 & NIP C8 showed Poly dispersity index below 0.3 which shows good uniformity in the droplet size distribution. Time of emulsification was under grade A (visual assessment criteria), thermodynamic stability testing & accelerated stability study was performed for optimized batches. In this study SMEDDS formed from Arachis Oil, Lutrol F-68, Triacetin & Propylene Glycol is a promising approach to improve the solubility, dissolution rate and bioavailability of Acetazolamide.

Keywords: Acetazolamide, Self micro emulsifying drug delivery system, Arachis Oil, Lutrol F-68, Triacetin, Droplet size.