Abstract

CHARACTERIZATION AND VALIDATION OF SILVER NANOPARTICLES FROM HOLARRHENA PUBESCENS – AN IMPORTANT ETHNOMEDICINAL PLANT OF KURNOOL DISTRICT, ANDHRA PRADESH, INDIA

*Venkata Subbaiah KP and Savithramma N

ABSTRACT

Holarrhena pubescens bark are extensively using by the ethnic groups of Kurnool district, Andhra Pradesh, India to cure vitiligo. Biological synthesis of silver nanoparticles was carried out from bark aqueous extract of Holarrhena pubescens 10 ml bark extract was mixed to 90 ml of 1 mM aqueous of Ag(NO3)2 and was heated at 60-800C for 20 min. The color change of aqueous solution into dark brown color. For characterization using UV-Vis spectrophotometer and AFM. AFM and UV-Vis spectrophotometer showed the formation of silver nanoparticles with spherical shape and average size 30.46 nm. SNPs have good antimicrobial activity against different microorganisms.

Keywords: Medicinal plant; Silver nanoparticles; Atomic Force Microscope (AFM); Inhibition zone; Secondary metabolites; Anti microbial efficacy.