Abstract

SIMULTANEOUS ESTIMATION OF LOTEPREDNOL ETABONATE AND TOBRAMYCIN IN THEIR COMBINED DOSAGE FORM BY RP-HPLC METHOD

L. R. Gandhi* and Ashish Kumar Sharma

ABSTRACT

A simple, rapid, precise, and accurate, reversed phase high performance liquid chromatographic method was developed and validated for simultaneous determination of Loteprednol etabonate and Tobramycin. The chromatographic separation was achieved on Grace 4.6 (id) x 250 mm column at detector wavelength of 230 nm, using an isocratic mobile phase consisting of acetonitrile and water in a ratio of 60:40 at a flow rate 0.8ml/min. The retention time for Loteprednol etabonate and Tobramycin were found to be 4.4 and 8.0 respectively. The method showed adequate precision with a relative standard deviation (RSD) smaller than 3%. The accuracy was analyzed by adding a standard drug and good recovery values were obtained for all drug concentration used. The HPLC method developed in this study showed specificity and selectivity with linearity in the working range and good precision and accuracy, making it very suitable for quantification of Loteprednol etabonate and Tobramycin in ophthalmic solution. The analytical procedure is reliable and offer s not only advantages in terms of speed but also low cost of reagents.

Keywords: Loteprednol etabonate, Tobramycin, Acetonitrile, Methanol etc.