Abstract

IN-VITRO AND IN-VIVO SCREENING OF ANGIOTENSIN CONVERTING ENZYME INHIBITOR USING MANGIFERA INDICA; A SOLUTION FOR HYPERTENSION

Radha Raman Raj*, Abha Kumari and Guruprasad

ABSTRACT

The angiotensin converting enzyme (ACE) functions a notable role in directing the blood pressure. By using hippuryl-L-histidyl-L-leusin as a substrate, the activity of ACE was screened between the eye and the heart of the zebrafish where eyes reveal highest activity. The screening of ACE activator is done from a plant methanol extract i.e. Mangifera indica. The max ACE activity is displayed by Mangifera indica. The GC-MS analysis of Mangifera indica detects 32 assorted compounds whereas Butanoic acidpropyl ester (CAS) Pro (13.60%), 9,12-Octadecadienoic acid (10.29%), methyl est, 9-Octadecenoic acid (Z)- (CAS) Oleic (9.53%), 2-Hydroxy-4-methylbenzaldehyde (9.47%) are in maximum concentration. All 32 compounds were placed to the docking studies with an ACE enzyme using the software Auto dock. Among these compounds, Urs-12-eneproves maximum affinity nearer the enzyme and this compound can be used as a potent ACE activator.

Keywords: ACE activator, Hypertension, Zebrafish, Mangifera indica, GC-MS, Urs-12-ene, Auto Dock.