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World Journal of Pharmacy and
Pharmaceutical Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology

Impact Factor : 8.025

ICV : 84.65

WJPPS Citation

	All	Since 2019
Citation	5450	3969
h-index	23	20
i10-index	134	84

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WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

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WJPPS: NOVEMBER ISSUE PUBLISHED

NOVEMBER 2024 Issue has been successfully launched on 1 NOVEMBER 2024.

Abstract

DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF KETOTIFEN AND SALBUTAMOL IN BULK AND DOSAGE FORM

*Raval Kashyap, U. Srinivasa

ABSTRACT

A simple, accurate and precise High Performance Liquid Chromatographic (HPLC) method has been developed for simultaneous determination of Ketotifen and Salbutamol in bulk and dosage form. The method has been validated as per the guidelines of ICH. The separation is achieved on 250*4.6mm C18, 5micron (Hypersil BDS) column with flow rate 1.0 mL per minute in isocratic mode using Buffer pH 3.5(0.1% triethylamine): Acetonitrile(60:40) as mobile phase. Column oven temperature is maintained at 25°C. The linearity range was found to be in the range of 5-15 μg/ml for Ketotifen and 10-30 μg/ml for Salbutamol. Correlation co-efficient for calibration curve of Ketotifen and Salbutamol was found to be 0.9988 and 0.9989 respectivelyThe method is simple, accurate, reproducible and short and can be used for simultaneous analysis of Ketotifen and Salbutamol.

Keywords: Ketotifen, Salbutamol, Buffer pH 3.5 and Acetonitrile.

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