Abstract

RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF IBUPROFEN AND FAMOTIDINE IN PHARMACEUTICAL DOSAGE FORM

T. Venkatakishore*, Dr. M. Prasadrao, B. Thulasi, K. Ambica, K. Mohan Satya Saikumar and G. Jaswanth

ABSTRACT

In this study, reversed phase high performance liquid chromatographic method have been developed and validated for the simultaneous determination of ibuprofen and famotidine in combined pharmaceutical formulation. In RP-HPLC method, optimization of chromatographic parameters was done. Optimization conditions was found to be 0.1% orthophosphoric acid (pH-3), acetonitrile in 50:50 v/v ratio as mobile phase at a flow rate of 0.8 mL/min. The peaks were well resolved with C18 column (46mm x 150mm , 5um) with a retention times 2.473 and 3.407 min for ibuprofen and famotidine respectively. UV detection was performed at 231 nm. The responses were linear (R2 > 0.9999) in the range of 80 – 400 μg/ml for ibuprofen and 2.6 – 13 μg/ml for famotidine. The % recovery for ibuprofen and famotidine was found to be 100.7 and 98.3 respectively. LOD and LOQ was found to be 0.121 μg/mL, 0.404 μg/mL for famotidine and 3.332 μg/mL, 11.1 μg/mL for ibuprofen respectively. No chromatographic interference from the tablet excipients was found. The results of the studies showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which can be applied for the routine analysis of ibuprofen and famotidine in tablet dosage forms.

Keywords: High performance liquid chromatography, validation, ibuprofen, famotidine and tablet dosage form.