Abstract

PROTECTIVE ROLE OF SOME NUTRACEUTICALS IN ALLEVIATING HEPATORENAL INJURY INDUCED BY CHEMOTHERAPY IN ADULT FEMALE RATS

Nashwah I. Zaki*and Lobna A. Hassanin

ABSTRACT

Nutraceuticals that exhibit antioxidant and or anti- inflammatory may aid in reducing organs injury induced by chemotherapy. Ifosfamide (IFO) is an alkylating oxazaphosphorine antitumor prodrug. Although it is an effective chemotherapeutic agent, it has been shown to induce many side effects. This study is performed to evaluate the ability of lecithin and/or quercetin to attenuate ifosfamide toxicity. Forty-nine female albino rats were randomly divided into seven groups. Group 1: Control, Group 2: Lecithin (100mg kg-1), Group 3: Quercetin (50mg kg-1), Group 4: IFO (80mg kg -1), Group 5: Lecithin plus IFO, Group 6: Quercetin plus IFO, Group 7: Lecithin and Quercetin plus IFO. Ifosfamide was administrated intraperitoneally, while lecithin and quercetin were given orally. The duration was six consecutive days for lecithin and quercetin and five consecutive days for IFO. Lecithin and quercetin singly reduce the severity of hepatorenal toxicity by restoring albumin, phosphorus levels and ALT activity near the control values (P<0.05). Also, they significantly improved tissue glutathione depletion, the rising in glutathione-S-transferase and myeloperoxidase activities, lowered the elevation of malondialdehyde and nitric oxide levels induced by ifosfamide in tissues (P<0.05). Combined treatment revealed more protective effects in serum and liver oxidative stress parameters including glutathione and malondialdehyde; as well as tissue gene expression for tumor necrosis factor-α, interluken-6, inducible nitric oxide synthase and DNA damage by comet assay (P<0.05). Conclusions: the current results suggest potential antioxidant, inhibiting neutrophil infiltration, cytokine response and oxidative DNA damage roles for lecithin and quercetin in reducing ifosfamide hepatorenal injury.

Keywords: Ifosfamide side effects - lecithin – quercetin – antioxidants - DNA damage by comet assay - iNOS, TNF-?, and IL-6 gene expression.