Abstract

SOME PHARMACOLOGICAL AND TOXICOLOGICAL ACTIVITIES OF ANNONA SQUAMOSA LINN. ETHANOLIC EXTRACT

El Banna, H.*, Ramadan, A., Elzorba, H. and Sayed, F.

ABSTRACT

The present study was to investigate phytochemical, toxicological and some pharmacological activities include antipyretic, analgesic and anti-inflammatory activity of Annona squamosa Linn.70% leaves in doses of 250 mg/kg. b. wt. and 500 mg/kg b. wt. and seeds in doses of 25 mg/kg b. wt. and 50 mg/kg b. wt. The phytochemical screening was done for detection of alkaloids, tannins, saponnin, flavonoids, glycosides and resin. The toxicological pattern was studied by the determination of LD50 in mice by oral administration of upgrading doses of leaves and seeds extract. The oral anti-inflammatory (Formalin induced edema), antipyretic (Brewer‟s yeast induced hyperthermia) were carried out on rats and analgesic activity (writhing test and hot plate method) were carried out on mice. Phytochemical screening revealed the presence of alkaloids, flavonoids and glycosides in both leaves and seeds. Resin was absent in leaves, seeds were devoid of tannins and saponin. LD50 was found to be 5000 and 520 mg/kg b. wt. for leaves and seeds respectively. Leaves exhibited significant analgesic and anti-inflammatory activity in doses of 250 and 500 mg/kg b. wt. and only higher dosage level (500 mg/kg b. wt) induced significant antipyretic activity. Seeds only exhibited significant analgesic and anti-inflammatory activity in a dose of 50 mg/kg b. wt. In conclusion, Annona squamosa leaves and seeds extracts induced antipyretic, analgesic and anti-inflammatory actions and leaves extract is consider more safer as compared with On the other seed extract.

Keywords: Annona squamosa, Phytochemical, Toxicological, LD50, Antipyretic, Analgesic, Anti-inflammatory.