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MOLECULAR CHARACTERISATION OF CLOSTRIDIUM PERFRINGENS ISOLATED FROM SHEEP USING MICRO-PCR, ELISA AND MALDI-TOF
Sobha Rani.M, BeenaRavi, Suhas.V. Bhandari, Venkatesh. M. D. and Asha Mayanna
ABSTRACT
Enterotoxaemia is a fatal and severe disease of sheep and goats caused by bacteria called Clostridium perfringens – types C and D. They release toxins into the blood stream and over multiply in numbers, which leads to sudden death of the cattle. Till date the diagnosis of this disease is purely based on traditional methods of identification of the bacteria. Using modern technologies in disease diagnosis not only helps in saving time, but also the lives of animals. In the present study both the traditional methods and modern methods like MALDI and ELISA were compared for detection of causative bacteria from the clinical samples. Using the conventional gram staining method and biochemical tests, the isolated bacteria have been identified as Clostridium perfringens. MALDI-TOF analysis also confirmed the presence of Clostridium perfringens. Using ELISA, the sub-types of Keywords: Enterotoxaemia, Clostridium perfringens, MALDI, ELISA. [Download Article] [Download Certifiate] |