Abstract

STUDIES ON PRODUCTION OF ANTIOXIDANT AND ANTI-PROLIFERATIVE SECONDARY METABOLITES FROM HALOPHILIC BACILLUS CEREUS ISOLATED FROM OPEN SOLAR SALT PANS

Venkatajalapathi P., Baskar M., Rajesh V., Sharmal Kumar M., Saraswathi K., Arumugam P. and Ravi Kumar M.*

ABSTRACT

The main aim of the study was to isolate marine bacteria which are antagonistically potent to produce the secondary metabolites having various industrial applications such as antioxidant and anticancer activity. The soil samples were collected from Marakkanam regions and five active strains were isolated. Among the five isolates (ABTRI 1-5) the strain ABTRI 2 was found to be antagonistically active for it’s antioxidant potential and the bioactive compounds were produced by ethyl acetate extraction method. The potent isolate was identified to be Bacillus sp. by 16srRNA sequencing. The anti-proliferative activity of the secondary metabolite of the isolate was evaluated on MCF-7 cell line by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The in vitro cytotoxicity assay on MCF-7 revealed that the bioactive compound had cytotoxic effect with IC50 31.11μg/ml.

Keywords: Marine Bacteria, DPPH assay, Thin layer chromatography, Bacillus sp., MTT assay.