ISOLATION, PURIFICATION AND CHARACTERIZATION OF AMYLASE FROM AIRBORNE-BACTERIA
Shivaji Bole*, Anindita Maji, Ankita Dey, Ashutosh Acharya, Siddharth Dubey,Rakeshlal
ABSTRACT
Among different types of enzymes obtained from microbial sources,
amylases are the most widely used in industries. In the present study,
bacteria were isolated from air exposure and screened for the
production of amylase. Among four bacterial isolates, one isolate
produced maximum zone of starch hydrolysis. The bacterial isolate
was identified as Bacillus sp. and was later used for further
characterization. Maximum yield of amylase was obtained after 48hrs
of incubation. The optimum pH for enzyme activity was found to be at
pH 6.8 and the optimum temperature for the activity was found to be at
37 C.
Keywords: Amylase, Starch Hydrolysis, Cultural characterization, Amylase activity, pH, Temperature.
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