Abstract

QUERCETIN PRODUCTION FROM HAIRY ROOT CULTURES OF INDIAN MALLOW (ABUTILON INDICUM L.)

Ramgopal Rao Sajjalaguddam* and Anitha Paladugu

ABSTRACT

The development of an efficient protocol for successful hairy root induction by Agrobacterium rhizogenes is the key step toward an in vitro culturing method for the mass production of secondary metabolites. The selection of an effective Agrobacterium strain for the production of hairy roots is highly plant species dependent and must be determined empirically. Therefore, in the present study, an attempt was made to investigate the transformation efficiency of two strains of A. rhizogenes for the induction of hairy roots in Abutilon indicum. In vitro synthesis of quercetin from hairy roots cultures of Abutilon was evaluated. Among MTCC 532 and 2364 strains used, strain 532 was most promising for hairy root induction with high growth rate, root number and root length. Hairy roots produced by 532 strain also exhibited higher quercetin content than 2364 strain. TLC and HPLC analysis of Abutilon hairy roots confirmed the presence of quercetin.

Keywords: Abutilon indicum, Quercetin, HPLC, Agrobacterium rhizogenes, Hairy root culture.