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DEVELOPMENT AND VALIDATION OF VISIBLE METHOD FOR ESTIMATION OF CEPHALEXIN IN BULK FORMULATION
Ch. Bhargavi, G. Rohit Reddy* and VVS. Rajendra Prasad
ABSTRACT A simple, sensitive, accurate, precise and economical visible Spectrophotometric method was developed and validated for the estimation of Cephalexin in Bulk form. The method is based on the reaction of Cephalexin with FC Reagent [Folin Ciocalteu] in the presence of 10% Sodium Carbonate giving greenish blue colour chromogen which shows maximum absorbance at 720nm against reagent blank. The Chromogen obeyed Beer’s law in the concentration range of 10-50 μg/ml for Cephalexin. The results of the analysis have been validated statistically and recovery studies. Keywords: Cephalexin, Folin Ciocalteu(Fc)reagent, Visible Spectrophotometric. [Download Article] [Download Certifiate] |