Abstract

ASSESSMENT OF THE IMPACT OF LEAF AND ROOT EXTRACTS OF THOTTEA SILIQUOSA (LAM.) DING HOU. A MEDICINAL HERB AS MICROBICIDAL

Saiba A.N, Gopi. T.V and Murugan.K*

ABSTRACT

Plants are utilized globally as herbal medicine due to their therapeutic values. Screening of plants for biologically active lead compounds against human pathogens is a renewed interested research in pharmaceutical science. In this study, Thottea siliquosa (Lam.) Ding Hou. a medicinal plant was evaluated in terms of in vitro microbicidal potentiality against selected bacteria and fungi. The species selected for the present study includes: Faecal streptococci, Pseudomonas aeruginosa, Lactococcus lactis, Rhodococcus equi, Escherichia coli, Salmonella typhimurium, Shigella flexineri, Vibrio cholerae, Vibrio parahaemolyticus, Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis, Klebsiella pneumoniae (bacterial) and five fungal species viz. Aspergillus niger, Aspergillus flavus, Trichoderma reesei, Candida albicans and Penicillium chrysogenum using different solvent extracts by hot continuous soxhlet extraction method. Petroleum ether and ethyl acetate extracts of T. siliquosa displayed potential antimicrobial activity. Phytochemical analysis reveals the presence of phenols, saponins, and alkaloids significantly. Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli and Staphylococcus epidermidis were significantly more susceptible while Faecal streptococcus, Lactococcus lactis, Rhodococcus equi, Salmonella typhimurium, Shigella flexineri, Vibrio cholerae, Vibrio parahaemolyticus were more resistant (P < 0.05).Meanwhile, in the case of fungi all the tested species are comparatively resistant except Candida albicans. The MIC and MBC/MFC are also significant. The data was further supported by time kill and spore germination assay.

Keywords: Thottea siliquosa (Lam.) Ding Hou., Microbicidal potentiality, Solvent extract, time kill assay, spore inhibition assay.