Abstract

PHYTOCHEMICAL SCREENING AND EVALUATION OF IN-VITRO ANTIBACTERIAL AND ANTHELMINTIC ACTIVITIES OF SACCHARUM OFFICINARUM LEAF EXTRACTS

M.N.Palaksha*, K.Ravishankar and V. Girija Sastry

ABSTRACT

The present investigation highlights the antibacterial and anthelmintic activities of Saccharum officinarum leaf extracts. The shade dried leaves were coarsely powdered and successively extracted with ethyl alcohol and water. The extracts were used to screen for the presence of phytochemicals followed by in-vitro antibacterial and anthelmintic activities. The different concentrations of water and ethyl alcohol extracts (50mg/ml and 100mg/ml) against different Gram negative (Pseudomonas putida ATCC700007 Pseudomonas mirabilus ATCC14153, Pseudomonas aeruginosa ATCC10662) and Gram positive (Bacillus subtilis ATCC11774, Staphylococcus wernari ATCC 27836, Staphylococcus aureus ATCCBAA1026) organisms using well diffusion method, was observed and the results were compared with standard Gentamycin, all the extracts were found to possess significant antibacterial activity. Different concentrations of leaf extracts were also tested to evaluate anthelmintic activity using Pheritima posthuma (Indian earthworm). Both the extracts were found to possess good vermifuge and vermicidal activity with 50 and 100mg/ml concentration and the results were compared with standard drug Albendazole.

Keywords: Saccharum Officinarum, Phytochemical Screening Antibacterial and