Abstract

INHIBITORY STUDIES OF SOME SELECTED PLANT PRODUCTS AS METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS (MRSA)

Suhas Souri J., Ashish Kumar Sharma* and Gaurav Sharma

ABSTRACT

Methicillin resistant staphylococcus aureus (MRSA) has been emerging as an important pathogen worldwide causing both, hospital acquired and community acquired infections. The study included the Efficiency and drug-likeness of plant compounds using pre-admet software. Various samples of MRSA were collected and tested included Pus, Wound swab, Blood, and Urine samples. Detection of Methicillin resistance strains were streaked on Mueller Hinton Agar with 4% NaCl for Oxacillin resistance. Anti-MRSA test was Performed using Kirby Bauer disk diffusion method on Muller Hinton agar plate on all of the isolates and interpreted using CLSI guidelines. Antibiotics used include Penicillin, Cefoxitin, Linezolid, Teicoplanin and Vancomycin. Microscopic examination revealed that the bacterial isolates were Staphylococci as the colonies were in clusters and Gram +ve, as they absorbed crystal violet. On MSA medium, fermentation of Mannitol resulted in the formation of yellow hollow around their growth. Colour of the colony was golden yellow. The isolates were catalase +ve, Coagulase +ve, DNAse +ve and hemolysis on Blood Agar plates was of beta type. On the basis of above characteristics all the bacterial isolates were identified belonging to genus Staphylococcus and species aureus and Methicillin Resistant Staphylococcus aureus (MRSA). This MRSA selected for the present study was inhibited by the isolated plant products. The strain was resistant to Penicillin, Cefoxitin and Oxacillin. It was sensitive to Vancomycin, Linezolid and Teicoplanin. Based on the results, conjugated linoleic (18:2) acid, thymoquinone, nigellone (dithymoquinone), melanthin, nigilline, damascenine and tannins, the plant product showing the maximum inhibitory of MRSA. It is to be seen if the docking values agree with the invitro anti bacterial activity of the plant products. If, the invitro experiments show good results, the plant products can be selected for use as potential anti- MRSA agents, which will also be of great benefit in combating antibiotic resistance of S.aureus causing serious human infection. Hence, this study evaluates the efficiency of the derivatives of spirohetero compounds as potential candidates of antimicrobial therapy.

Keywords: MRSA, Spirohetero compounds, Vancomycin, Damascenine.