Abstract

EX-VIVO PROTEIN BINDING STUDY OF CEFIXIME BY USING DIFFERENT MEMBRANE

L. Subramanian*, M. Ramanathan, S. Amritha, G. Kalaivani, M. Maheswari, P. Solairai

ABSTRACT

Drug efficiency may be affected by blood plasma protein binding. The less bound drug is more effective; it can traverse cell membranes or diffuse. Protein binding can influence the drug's biological half-life in the body. The bound portion may act as a reservoir from the drug is slowly released as the unbound form. Cefixime is a third generation cephalosporin antibiotic. It is used in the treatment of typhoid fever, uncomplicated urinary tract infection and otitis media. Conventional cefixime dosage form shows absorption and permeability problem, it reflects in bioavailability. In the present work was an attempt to determine the permeability of cefixime by protein binding study using different membrane (cellophane membrane and egg membrane). Drug protein complex using the above said two membranes. The Drug protein complex showed around 63 % ex-vivo drug release at the end of 60 min, the pure drug (without protein) release is 91 %. These results revealed that the drug diffuse can be determined by incorporation with protein (egg albumin).

Keywords: Cefixime, Drug protein complex, Cellophane membrane, Egg membrane, Ex-vivo drug release.