Abstract

PHYTOCHEMICAL EXTRACTION AND TLC ESTIMATION OF EXTRACT OF CUSCUTA REFLEXA (DODDER)

Dr. Kalpana Teware*

ABSTRACT

Cuscuta (dodder) is a genus of about 100–170 species of yellow, orange, or red (rarely green) parasitic plants. Formerly treated as the only genus in the family Cuscutaceae, it now is accepted as belonging in the morning glory family, convolvulaceae, on the basis of the work of the angiosperm phylogeny group. In preliminary phytochemical evaluation, different solvents systems were tried for developing a TLC system for phytochemical investigation of Cuscuta reflexa. In this piece of work dried powdered whole plant Cuscuta reflexa has been extracted with Ethanol, pet ether and Aqueous using Soxhlet’s apparatus for 48 hrs, filtered and dried using vacuum evaporator at 400C. Yield are obtained as in pet ether, ethanol and aqueous extract of experimental plant is 1.80%, 3.56%and 4.25 % respectively. Comparatively phytochemical investigation revelas the presence of phenol compounds, flevenoides, and terpenoides in ethanolic and aqueous extract. TLC study of these shows the presence of gallic acid and quarcetin in ethenolic extract of Cuscuta reflexa at Rf value 0.65 and 0.82. Presence of phenolic and flevenoides shows that this plant can be use for various pharmacological studies in. betterment of human life.

Keywords: Morphology, Cuscuta reflexa, ethanol, phytocemical investigation, TLC.