Abstract

GAS CHROMATOGRAPHIC AND MASS SPECTROMETRIC ANALYSIS OF PHYTOCHEMICALS PRESENT IN FROND AND RHIZOME METHANOLIC EXTRACT OF DRYNARIA QUERCIFOLIA (L.) J. SM.

Saurabh H. Patil*, Vikas V. Vaidya, Deepal D. Ghadge and Pushkar M. Pradhan

ABSTRACT

Drynaria quercifolia (L.) J. Sm. commonly known as Oak-leaf fern is a pteridophyte belonging to the family Polypodiaceae with wide spread ethno-pharmacological uses. The present study reports the gas chromatography-mass spectrometry method for the fingerprinting analysis of phytochemicals present in the methanolic extract of fronds and rhizomes of Drynaria quercifolia. GC-MS analysis was performed using Shimadzu GCMS-QP2010 system. Rtx-5ms column was used for the experiment and phytochemicals were separated using temperature programmed run. The mass spectra of the compounds found in the extract were matched with the National Institute of Standards and Technology (NIST) library. The GC-MS analysis has shown the presence of 34 compounds in the frond and 67 compounds in the rhizome of Drynaria quercifolia including alkaloids, flavonoids, triterpenoids, phytosterols, tannins, saponins and fatty acids. Further studies are needed to isolate the active components of the extracts as well as to elucidate their exact mechanism of action against various disorders.

Keywords: Drynaria quercifolia (L.) J. Sm., Polypodiaceae, Gas Chromatography-Mass Spectrometry, Fingerprinting.