Abstract

CHEMICAL COMPOSITION OF ESSENTIAL OILS FROM IN VITRO CULTURED RUTA GRAVEOLENS L.

Ajit A. Kengar* and Govind T. Paratkar

ABSTRACT

In vitro shoots obtained from bioreactors and the fresh leaves of tissue cultured hardened plant of Ruta graveolens were used for extraction of oil. The essential oils were recovered by hydro-distillation with different percentage yield. The obtained essential oils were analyzed by GC–MS.The oil composition showed variation in terms of number of component and their percentage. The number of compound found in the oil obtained from fifteen days in vitro cultures shoots grown simple aerated bioreactor were more than 30 days bioreactor shoots and tissue cultured hardened plant shoots. The essential oils from field grown micro propagated plants of Ruta graveolens, when compared with those from bioreactor shoots of the same clone, showed that the same major components were present with significant compositional variations. The main composition of analyzed oil showed percentage of 2-Heptanone, was more in 15 days bioreactor shoots than other and 2-Undecanone, 2-Nonanone, etc was main components of 30 days bioreactor shoot and tissue cultured hardened shoot oils.The identification of the components in vitro shoots of R.graveolens essential oil should contribute to the understanding biosynthesis pathway of oil.

Keywords: Essential oils, Ruta graveolens, GC–MS, Bioreactors, 2-Undecanone.