Abstract

ANTIBACTERIAL EFFECTS OF AERIAL PART OF WITHANIA SOMNIFERA WITH CHLOROFORM EXTARCT ON PATHOGENIC STRAINS

Priyanka Kumari Panchal*, Nishi Sharma and Kamal Singh

ABSTRACT

The present study was planned to investigate the microbial inhibitory effects of Withania somnifera leaves. The preliminary successive solvent extraction and chemical test revealed the presence of secondary metabolites in various solvent extracts. Microbial inhibitory effects has provide us clue for further investigation. Chloroform extract was used for investigation of inhibition on five pathogenic bacterial strains and done by broth dilution methods with triplicate. Tetracycline used as positive control and double distilled water was used as Negative control. Statistical analysis was done by Mean ± Standard Deviation with range, in which n=5 and one way ANOVA done with SPSS 20.0, in which p values were considered significant at 95% confidence intervals (p

Keywords: Withania somnifera, secondary metabolites, antimicrobial activity, chloroform, total activity, statistical analysis, Phytoconstituents.