Abstract

FLOW-INJECTION CHEMILUMINESCENCE ANALYSIS OF MINOCYCLINE HYDROCHLORIDE IN CAPSULES AND SERUM

Ting Wang, Peiyun Chen, Ming Su, and Hanwen Sun*

ABSTRACT

A new flow–injection chemiluminescence(CL) method was developed for the determination of minocycline hydrochloride. The enhanced effect of minocycline hydrochloride on cerium(IV)–rhodamine 6G CL emission was investigated based on the studies of kinetic process and the CL spectra, and a CL mechanism was proposed to be due to that rhodamine 6G and minocycline hydrochloride are oxidized by cerium(IV) to form the excited-state cerium(III), which transfers energy to rhodamine 6G, resulting in light emission. The CL conditions were investigated and optimized. The CL intensity was correlated linearly with concentration of minocycline hydrochloride in the range of 5.07×10‒8 ~1.42×10‒5 g/mL, with a correlation coefficient (r) of 0.9996. The detection limit (3σ) is 5.05×10‒8 g/mL. The relative standard deviation is 1.1% for 11 measurements of 3.07×10‒6 g/mL minocycline hydrochloride. The method has been applied satisfactorily to determine the drug in capsules and spiked serum sample with the recovery of 82.03‒110.5% with RSD of 0.8‒1.8%.

Keywords: flow-injection, chemiluminescence, minocycline hydrochloride.