Abstract

MALDI-TOF MASS SPECTRUM PROFILING, ANTIBACTERIAL AND ANTICANCER ACTIVITY OF MARINE STREPTOMYCES FRADIAE BDMS1

Sankaran Ganesan*, Govindasamy Velsamy, Thilagar Sivasudha,Natesan Manoharan

ABSTRACT

To obtain the carbohydrate biomolecules present in the dichloromethanolic extract of marine Streptomyces fradiae strain BDMS1 by different chromatography and spectroscopic analysis of its antibacterial and anticancer activity. Marine actinobacterium isolate from the surface sediment from Thondi regions in southeast coast of India. The isolate of actinobacteria was identified based on the scanning electron microscopic and molecular characteristic. Further, antibacterial and anticancer activity of dichloromethane extract was evaluated against twelve pathogenic strains by disk diffusion methods and cytotoxic assay was tested at several concentrations (10 to 80μg/ml). A marine isolated strain was identified by using 16S rRNA partial gene sequence has confirmed its membership to the species Streptomyces fradiae. The presence of aminoglycosyl functional groups was revealed by Fourier Transform Infrared Spectrophotometer (FT-IR) analysis. The identification of aminoglycoside biomolecules of the dichloromethane extract was done by different chromatography and spectroscopic analysis. Seven compounds were identified of which two fradimycin C and urdamycin A showed maximum intensity of the peak. The dichloromethane extract showed potent antibacterial and anticancer activity. We conclude, Streptomyces fradiae BDMS1 was found to have aminoglycoside biomolecules and it is essential to identify the compounds responsible for antibacterial and anticancer activity.

Keywords: Streptomyces fradiae, Aminoglycoside, Fradimycin C, Antibacterial, Anticancer