Abstract

MODULATION OF GUT MICROBIOTA DURING PROBIOTIC-MEDIATED LEAD TO REDUCTION OF BODY WEIGHT IN IRAQI OBESE FEMALE.

Fakhri S. Alajeeli* and May T. Flayyih

ABSTRACT

Gut flora play important role in regulation of body weight and all study share that is may be used to safety treatment of obesity. Methods: 25 healthy obese female volunteers was participated in this study and divided in two group, G1 used probiotic with 4 strain one capsule per day and G2 was used placebo capsule both group used same diet program for 8 weeks, stool sample was collected before and after study finished and extracted DNA form stood and used q PCR technology by sued specific primers. Results: Microbial abundance measured by quantitative polymerase chain reaction (qPCR), the Bacteroids spp. showed significant increased in probiotic group G1 in initial of study it was percentage 36% and after consumed probiotic it was increased to 42% compared with placebo groups G2 it showed decreased 33%. In Ruminocococus spp, the probiotic group decreased to 18% compared with baseline 23%, but in the placebo group the percentage was still 23% without any change In the Bafidobacterium spp. Bifidobacterium was consist of 0.05% and in the probiotic group G1 it was increased to 24% and in the placebo group G2 its decreased to 0.01%.the Bifidobaiterium spp. Show highly significant p≤0.0001.The lactobacillus spp. showed significant deference with p≤ 0.026 it showed increased to 11% in the probiotic group compared with baseline (0.005)% and compared with placebo group it was decreasd to 0.004% . Bacteroidetes to Firmicutes (B/F) ratio was increased in G1 group( 2.33) compared with placebo group (1.4) and in baseline the ratio was (1.56). In the body weight The group(G1) who consumed probiotic showed decrease (5.4%) 5.4kg from (92.92) to (87.88) and the placebo group showed decreased 4.14% (3.4)kg from (91.28) to (87.5) but there was no significant difference between two groups.

Keywords: Bacteroids, Bafidobacterium, Ruminocococus.