Abstract

TOTAL PHENOLIC AND TOTAL FLAVONOID COMPOUNDS IN THE LEAVES OF ROYLEA CINEREA USING HYDROALCOHOLIC EXTRACT

Shweta Bisht*, Jaya Pangti, Swati Pundir, Anju Das, Jyotsana Devi5

ABSTRACT

Roylea cinerea D.Don Baillon belongs to the family Lamiaceae. It is a shrub of monotypic genus and found in western Himalayas from Kashmir to Nepal at an altitudes from 600-1500 m. Synonyms of Roylea cinerea are Roylea elegans and Roylea calycina. Vernacular name of R.cinerea may include Patkarru, Titpati, Karanoi, Karui, Kaur and Kauri. Whole plant parts are widely used as folklore medicine. Shoots are crushed and eaten with salt to strengthen the liver by local villagers. Young shoots are used as insect repellent for cattle during rainy season. Leaves and shoot extraction is used in scabs and other skin infections. Aerial parts are widely used to treat various liver disorders like jaundice, liver debility and fever. Pharmacognostical and preliminary phytochemical screening of leaves of Roylea cinerea (D.Don) Baillon were followed as per WHO guidelines. In Pharmacognostical screening macroscopic evaluation, microscopic evaluation, transverse section, physicochemical standardization methods were employed, whereas, in phytochemical screening successive solvent extraction, phytochemical tests were carried out. Gallic acid is used as the standard which represents the phenolic compound in the plant R.cinerea 10 mg of Gallic acid monohydrate dissolved in 100 ml of ethanol to give concentration of 100 μg/L. Quercetin is used as the standard for estimation of total flavonoids in the prepared extract. 10 mg of quercetin dissolved in 10 mL of methanol to get 1000 μg/mL solution.

Keywords: Jaundice, skin infections, phenolic content, flavonoid content, quercetin, gallic acid.