Abstract

ANTI-INFLAMMATORY EFFECT OF TEMPOL IN ORGANOPHOSPHATE POISONING

Mauricio Alexandre Reis Junior*, Fernanda Borges Araújo Paula, Maisa Ribeiro Pereira Lima Brigagão

ABSTRACT

The malathion is one of the most organophosphates used in agriculture. Several studies have pointed to the organophosphate malathion as a substance capable of causing effects mutagenic, carcinogenic and hepatotoxic associated with exacerbated production of reactive oxygen species and nitrogen. The objective of this study was to evaluate the nitroxide Tempol is capable of inhibiting activity phagocytic Nox2 complex and reduce the inflammatory process induced by organophosphate malathion. Furthermore, was evaluated if the nitroxide Tempol is able to reduce the hepatotoxic effects induced by poisoning after exposure to organophosphate malathion. To ensure a standardized methodology of exposure, the organophosphate malathion was quantified in plasma by gas chromatography. In this project wasevidenced that the Tempol nitroxide inhibited the translocation of p47phox in Nox2 complex, reducing oxidative stress induced by malathion. Parallel to this, it observed an increase of both transaminases, AST and ALT, after administration of malathion, indicating a subchronic exposure to organophosphate causes liver injury, exacerbated event by administration of Tempol. These results indicate that, although the nitroxide Tempol (anti-inflammatory prototype) is an efficient antioxidant, its metabolism concomitantly with malathion (organophosphate) can exacerbate liver damage.

Keywords: Inflammation, Malathion, Tempol, Organophosphate.