Abstract

IN VITRO BIOACTIVITY STUDIES OF WILD CURCUMA ANGUSTIFOLIA RHIZOME EXTRACT AGNAIST (HeLa) HUMAN CERVICAL CARCINOMA CELLS

Sanghamitra Nayak*, Alok Kumar Jena, Swati Sucharita

ABSTRACT

Purpose: To investigate the in vitro antioxidant, antimicrobial and anticancerous activity of curcuma angustifolia methanolic extracts a plant associate from Eastern Ghats of Odisha, India. Methods: Antioxidant activity of methanolic rhizome extract was evaluated in terms of total phenol content, ascorbic acid total flavonoids content, DPPH radical scavenging activity. Antimicrobial activity of plant extract was determined by agar well diffusion method against six bacterial strains including gram positive and gram negative bacteria. Cytotoxicity activity of plant extract was studied on cervical cancer cell line by MTT assay methods. Results: Antioxidant activity by DPPH scavenging activity posse’s strong antioxidant properties of (66.82±0.05) with good amount of ascorbic acid (1.802±0.282 mg/ml) and flavonoid (3.261±0.008 mg/ml) was found from methanolic extract of Curcuma angustifolia .Antimicrobial activity was evaluated by six pathogenic bacteria with both gram positive and gram negative bacteria, methanolic extract showed moderately effective against four strains and not effective against two pathogenic strain. Anticancerous activity of plant extract was tested on cervical cancer cell line (HeLa).At a concentration of 694.20 μg/ml, the extracts exhibited effective anticancer activity towards HeLa cells, at this concentration the extract of Curcuma angustifolia exhibit cell viability 65.55%. Moreover, towards HeLa treated with paclitaxel (positive control) showed 44.44% viability at same concentration (694.20 μg/ml).The IC50 values for HeLa cells are calculated was 78.47 μg/ml. Conclusion: The present study reveal that the methanolic rhizome extract of Curcuma angustifolia showed potential antioxidant, antimicrobial and anticancerous properties which could be exploited in preparation of herbal drugs with modern standard and safety.

Keywords: Wild Curcuma angustifolia; Cytotoxicity; Antioxidant; Antimicrobial; HeLa cell line.