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CLONING AND EXPRESSION OF NOVEL LINGO-1 GENE FROM BUBALUS BUBALIS
Imteyaz Qamar*, Nagendra Singh, Sahar Waseem, Prapti Prakash, Veerendra Singh Nagoria and Mohammad Faiz Ahmad
ABSTRACT
In this study, we have identified, cloned and expressed full length Lingo-1 gene from water buffalo Bubalus bubalis testis cDNA. LINGO-1 gene is well studied in various animal models. But it is a novel protein to be studied in Bubalus bubalis. Previous studies by other groups uncovered several transcripts representing known and novel genes tagged with consensus of 33.15 using cDNA from the testis, ovary, spleen, kidney, heart, liver and lung of water buffalo Bubalus bubalis. Among those transcripts one was found to represent the partial cDNA sequence of LRRN6A. Studies of transcripts using Minisatellite-Associated Sequence Amplification (MASA) showed the Keywords: Bubalus bubalis, Bos Taurus, cDNA, LRRN6A. [Download Article] [Download Certifiate] |