Abstract

IDENTIFICATION OF TERPENOIDS AND STEROIDAL COMPOUNDS IN CARYOTA URENS LEAVES BY COLUMN CHROMATOGRAPHY AND VARIOUS SPECTROSCOPIC TECHNIQUES.

Mariyan R. Patel*, Hiteksha S. Panchal and Ajay K. Saluja

ABSTRACT

The increasing interest in powerful biological activity of secondary metabolites outlined the necessity of determining their contents in medicinal plants. The present study intended to find out the active constituents. Caryota urens plant is rarely mentioned in published books and there is no data of showing which constituents are present in this plant Qualitative chemical examination of various successive extracts of leaves of Caryota urens showed presence of Phytosterols, triterpenoids, flavanoids, tannins and phenolic compounds and carbohydrates were present. Eluent and isocratic column chromatography was used for isolation of compounds. Isolation of terpenoids was done by column chromatographic technique, using different ratio of solvent from nonpolar to polar i.e., hexane, pet ether, toluene, chloroform, ethyl acetate and methanol as an eluent and two compounds were isolated. These compounds were terpenoids which are confirmed by liberman burchard’stest, salkowski’s test and TLC with Anisaldehyde in H2SO4 reagent. Spectroscopic data of UV, FT-IR, GC-MS, chemical shift of U.V gave the probability of monoterpenoids and sesquiterpenoids. Isolation of steroid was done by column chromatographic technique and that gave probability of steroidal moiety in the isolated compound. However this requires further LC-MS for the confirmation of compound.

Keywords: Caryota urens, Column chromatography, Spectrophotometric methods like UV, IR, GC, Mono terpenoids, sesquiterpenoids, Steroidal Moiety.