Abstract

SYNTHESIS AND EVALUATION O F POLYAMIDOAMINE (PAMAM) DENDRIMER AS A CARRIER OF CEFIXIME DRUG

Latha Subbiah*, Selvamani Palanisamy, Bharathi Thendral Sivaprakasam, Dharani Elamurugan

ABSTRACT

Dendrimers are Nano-sized polymer, radially symmetric molecules with well-defined, homogeneous and monodisperse structure. Apart from conventional methods of solubility enhancement, there are some novel approaches such as using dendrimers for increasing their solubilization. These properties make the dendrimers a suitable carrier in drug delivery applications. They can be used as novel nano carriers, solubility enhancers and for controlled release drug delivery system. PAMAM (polyamidoamine) dendrimers synthesized using ethylene diamine as initiator core and methyl acrylate as repeating unit. Synthetic process involves michael addition. The loading of cefixime drug was carried out and confirmed by UV spectra and changes in the absorption maxima of PAMAM dendrimers indicate the complex formation. The solubilty of drug in the presence of dendrimers was measuered at 37oC. The λmax values were found to be in range of 277 to 286 nm. IR spectrum peaks of N-H stretch for primary amine were obtained at 3310.21cm -1. The particle size of 4G and 5G is 4.55 and 5.05nm respectively. Antibacterial activity of cefixime against E.coli, Vibrio cholera, bacillus subtilus, Shigella flexneri in the presence of PAMAM dendrimers were efficient. It was observed that the low water solubility of cefixime has been significantly improved by PAMAM dendrimers (G5).

Keywords: Dendrimers, solubilizer, PAMAM, PEGylation.